|  Help  |  About  |  Contact Us

Allele : Col1a1<tm2(tetO-GFP/RNAi:Rpl5)Begg> collagen, type I, alpha 1; targeted mutation 2, Alan Beggs
Primary Identifier  MGI:6368192 Allele Type  Targeted
Attribute String  Inducible, Knockdown, Reporter Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Rpl5 into the Col1a1 locus. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a turboGFP sequence (tGFP; a fast-maturating and short-half-life green fluorescent protein variant) and short-hairpin RNA sequence targeting Rpl5 embedded in the MIR30 structure. The vector was then co-electroporated with a FLPe recombinase-expressing vector into the RMCE-ready KH2 ES cells.
  • mutations:
  • Insertion
  • synonyms:
  • shRNA Rpl5,
  • shRNA Rpl5
Paste the following link
Quick Links:
SummaryGenomePhenotypeOther
 
Quick Links:
SummaryGenomePhenotypeOther
 
Lists
This Allele isn't in any lists. Upload a list.

1 Feature

Trail: Allele

Genome

0 Expresses

1 Mutation Involves

Trail: Allele

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

3 Publication categories

Trail: Allele




MouseMine is a collaboration between MGI at The Jackson Laboratory and the InterMine project at the Cambridge Systems Biology Centre. MouseMine is funded through a grant from the NIH/NHGRI.The Jackson Laboratory makes no representation about the suitability or accuracy of this software or data for any purpose, and makes no warranties, either express or implied, including merchantability and fitness for a particular purpose or that the use of this software or data will not infringe any third party patents, copyrights, trademarks, or other rights. the software and data are provided "as is". This software and data are provided to enhance knowledge and encourage progress in the scientific community and are to be used only for research and educational purposes. Any reproduction or use for commercial purpose is prohibited without the prior express written permission of the Jackson Laboratory.

Copyright © 2017 by The Jackson Laboratory. All Rights Reserved

© 2002 - 2017 Department of Genetics, University of Cambridge, Downing Street,
Cambridge CB2 3EH, United Kingdom