Primary Identifier | MGI:6368782 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Stk33 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to replace the Stk33 locus between exon 6 and exon 8 with a cassette containing exon 7 of Stk33, a neomycin resistance gene flanked by FRT sites, and loxP sites surrounding exon 7 and the neomycin cassette. The neomycin cassette was removed through Flp-mediated recombination via crossing to ACTB:FLPe B6J mice to obtain the conditional strain that contains loxP recombination sites flanking exon 7 of Stk33. |