| Primary Identifier | MGI:6382787 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gtf2h3 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CCCATGGCAAAGAAGCTGAG and ATACACCAATTCCCACCACA, which resulted in a 695 bp deletion beginning at Chromosome 5 position 124,583,657 bp and ending after 124,584,351 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001266779 and ENSMUSE00001246016 (exons 3 and 4) and 421 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 31 and early truncation 25 amino acids later. |
