| First Author | Lee JH | Year | 2018 |
| Journal | Transgenic Res | Volume | 27 |
| Issue | 3 | Pages | 241-251 |
| PubMed ID | 29594927 | Mgi Jnum | J:265348 |
| Mgi Id | MGI:6199341 | Doi | 10.1007/s11248-018-0069-y |
| Citation | Lee JH, et al. (2018) Differences between immunodeficient mice generated by classical gene targeting and CRISPR/Cas9-mediated gene knockout. Transgenic Res 27(3):241-251 |
| abstractText | Immunodeficient mice are widely used for pre-clinical studies to understand various human diseases. Here, we report the generation of four immunodeficient mouse models using CRISPR/Cas9 system without inserting any foreign gene sequences such as Neo(R) cassettes and their characterization. By eliminating any possible effects of adding a Neo(R) cassette, our mouse models may allow us to better elucidate the in vivo functions of each gene. Our FVB-Rag2(-/-), B6-Rag2(-/-), and BALB/c-Prkdc(-/-) mice showed phenotypes similar to those of the earlier immunodeficient mouse models, including a lack of mature B cells and T cells and an increase in the number of CD45(+)DX-5(+) natural killer cells. However, B6-Il2rg(-/-) mice had a unique phenotype, with a lack of mature B cells, increased number of T cells, and decreased number of natural killer cells. Additionally, serum immunoglobulin levels in all four immunodeficient mouse models were significantly reduced when compared to those in wild-type mice with the exception of IgM in B6-Il2rg(-/-) mice. These results indicate that our immunodeficient mouse models are a robust tool for in vivo studies of the immune system and will provide new insights into the variation in phenotypic outcomes resulting from different gene-targeting methodologies. |
