| First Author | Couto J | Year | 2023 |
| Journal | STAR Protoc | Volume | 4 |
| Issue | 2 | Pages | 102303 |
| PubMed ID | 37178116 | Mgi Jnum | J:359527 |
| Mgi Id | MGI:7787259 | Doi | 10.1016/j.xpro.2023.102303 |
| Citation | Couto J, et al. (2023) Protocol for infecting and monitoring susceptible k18-hACE2 mice with SARS-CoV-2. STAR Protoc 4(2):102303 |
| abstractText | Intranasal infection of k18-hACE2 mice with SARS-CoV-2 recapitulates the clinical characteristics present in severe COVID-19. Here, we present a protocol for intranasal administration of SARS-CoV-2 to k18-hACE2 mice and their subsequent daily monitoring. We describe steps for intranasal inoculation of SARS-CoV-2 and the collection of clinical scores on weight, body condition, hydration, appearance, neurological symptoms, behavior, and respiratory movements. This protocol contributes to the establishment of a model of severe SARS-CoV-2 infection that minimizes animal suffering. For complete details on the use and execution of this protocol, please refer to Goncalves et al. (2023).(1). |
