Primary Identifier | MGI:6392027 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cd2bp2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 1. An additional loxP site was inserted downsteam of exon 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 1 through 3 floxed. Western blot analysis confirmed the absence of protein expression in embryos. |