| Primary Identifier | MGI:7564294 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Null/knockout | Gene | Pcdh15 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Arginine codon 250 (CGA) in exon 9 was changed to a stop codon (TGA) (p.R250*) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the human p.R245* mutation associated with Usher syndrome type I (USH1). Immunohistochemistry experiments indicate absence of protein expression in retinal and cochlear tissues. |
