| Primary Identifier | MGI:6393452 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Spast |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 5-8 were replaced with a loxP site, a puromycin cassette flanked by FRT F3 sites, exons 5-7, a neomycin cassette flanked by FRT sites, a loxP site, and an exon 8 containing a C to A mutation at position 1152 resulting in an asparagine to lysine substitution at amino acid 384 (N384K). Flp-mediated recombination removed the selection markers. Western blot analysis of fetal brain tissue confirmed expression of protein. |
