Primary Identifier | MGI:6393676 | Allele Type | Targeted |
Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | C57BL/6 and 129 |
Is Recombinase | false | Is Wild Type | false |
description | The allele was made in MESC10 ES cells that carry an FRT-PGK-neomycin-FRT (Flp in) site downstream of the mouse Col1a1 gene. |
molecularNote | A loxP-Stop-loxP-Human RHOA cDNA cassette, modified with a Y42C amino acid mutation (nucleotides TAT to TGT) in RHOA, was introduced to an intergenic region downstream of the mouse Col1a1 gene via Flp recombinase-mediated homologous recombination. |