| Primary Identifier | MGI:6393544 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dnaaf3 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTAACCCTAGGCCACAGTAG and TCTCTGAAAAATCTTGCCCA, which resulted in a 617 bp deletion beginning at Chromosome 7 position 4,527,750 bp and ending after 4,528,366 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000419696 (exon 5) and 459 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 107 and early truncation 21 amino acids later. |
