| Primary Identifier | MGI:6404032 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Ttk |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was designed to insert a loxP-flanked cassette containing cDNA encoding exons 17-22 from the gene downstream of the endogenous exon 16. The 3' loxP site was preceded by a stop codon and polyadenylation sequence. Downstream of this cassette, endogenous exon 17 was modified to harbor an ACA to GCA mutation at codon 649, resulting in a threonine to alanine mutation (T649A) similar to that found in human cell lines with a mild chromosomal instability (CIN) phenotype. An FRT-flanked puromycin cassette was also included and then removed by Flp-mediated recombination. |
