| Primary Identifier | MGI:6438113 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Shank3 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 4 to 9 were flanked by loxP sites and an FRT-flanked neomycin selection cassette was inserted downstream of the second loxP site. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination deleted exons 4-9 in the germline. Western blot analysis indicates loss of the two highest molecular weight isoforms (C-1, C-2, N-1, and N-2 bands) and a reduction in other isoforms (bands C-3, C-7, and N-3 on the blot) indicating a complex alteration of isoforms. |
