| Primary Identifier | MGI:6416147 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Pithd1 |
| Transmission | Germline | Strain of Origin | C57BL/6NJcl |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell = HK3i (C57BL/6NJcl) |
| molecularNote | A cassette containing a tdTomato reporter followed by a floxed pgk-neo gene in inverse orientation was inserted in-frame just after the start codon in exon 1 replacing part of exon 1. Quantitative RT-PCR and immunoblot analysis confirmed the absence of expression of wild-type RNA and protein in the thymus and testis of homozygous mice. Reporter expression was detected in the cortical region of the thymus in heterozygous mice. |
