| Primary Identifier | MGI:6415727 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified isoform(s) | Gene | Scn5a |
| Strain of Origin | FVB/NJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology was used to delete the alternate exon 6B and redirect splicing to exon 6A. This results in expression of only the fetal isoform. RT-PCR of heart tissue confirmed that homozygotes exclusively express Scn5a with exon 6A and heterozygotes have approximately 70% Scn5a with exon 6A. |
