| Primary Identifier | MGI:6436287 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mirc21 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The region containing Mir34b and Mir34c was flanked by loxP sites. The distal LoxP site was inserted into a non-conserved region approx. 400 bp downstream of Mir34c. A positive selection cassette (NeoR) was flanked by FRT recombination sites and co-inserted with the proximal LoxP site into non-conserved regions approx. 800 bp upstream of Mir34b sequence. The conditional allele was obtained after Flp-mediated removal of the selection marker. Cre-mediated recombination removed the microRNA cluster. |
