| Primary Identifier | MGI:6458812 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene insert comprises the following elements: the CAG promoter, a loxP site, the EGFP fluorescent reporter gene, poly(A) signal sequence, a loxP site, the mCherry fluorescent reporter gene, sequence coding for T2A self-cleaving peptide, the cDNA sequence for the mouse Dnmt3a2 short splice variant, sequence coding for T2A self-cleaving peptide, mouse Dnmt3l cDNA sequence, and poly(A) signal sequence. This allele expresses the EGFP gene; mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene. Two mouse lines, ak and q, carrying an estimated 8-10 copies of the transgene, were selected for further study. This allele uses the pound # sign for when the sub-line is not defined. |
