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Allele : Igs2<tm1(CAG-CD59)Bga> intergenic site 2; targeted mutation 1, Bin Gao
Primary Identifier  MGI:6469895 Allele Type  Targeted
Attribute String  Conditional ready, Humanized sequence, Inserted expressed sequence Gene  Igs2
Transmission  Germline Strain of Origin  (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Is Recombinase  false Is Wild Type  false
description  TARGATT mice carry the H11 allele ( Igs2tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted transgenic insertion of the construct into the first and second attP sites of the locus.
molecularNote  Site-specific PhiC31 integrase-mediated transgenesis is used to insert a chicken beta-actin promoter with a CMV enhancer (CAG promoter) followed by a loxP-flanked stop cassette and the entire open reading frame of the human CD59 gene into the intergenic site 2 locus. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice on a FVB/N congenic background.
  • mutations:
  • Insertion
  • synonyms:
  • ihCD59,
  • LSL-hCD59,
  • ihCD59,
  • LSL-hCD59
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1 Feature

Trail: Allele

Genome

1 Expresses

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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