| Primary Identifier | MGI:6504237 | Allele Type | Targeted |
| Attribute String | Inducible, Knockdown, Reporter, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Atg5 into the Col1a1 locus. D34 embryonic stem cells, expressing CAG-rtTA3 knocked into the Rosa26 locus, were used. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Atg5 embedded in the miR-E design (an improved design variant of Mir30 structure). |
