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Allele : Col1a1<tm1(tetO-GFP,-PRKAA1*)Rjsh> collagen, type I, alpha 1; targeted mutation 1, Reuben J Shaw
Primary Identifier  MGI:6472613 Allele Type  Targeted
Attribute String  Inducible, Inserted expressed sequence, Reporter Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The embryonic stem cell line used is (C57BL/6 x 129S4/SvJae)F1-derived D34 that had previously been targeted with a Col1a1 "Flp-In" integration cassette (Col1a1-FRT-hygro-pA) and Gt(ROSA)26Sortm2(CAG-rtTA3,-mKate2)Slowe.
molecularNote  The targeting construct includes a Tet-responsive element (TRE or tetO) directing expression of an eGFP reporter followed by an IRES and a truncated (amino acids 1-312) human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1 or AMPKalpha;1) cDNA. The construct, a "Flp-In" promoter cassette (PGK-promoter-ATG-FRT) and a pCAGGS-FlpE plasmid were electroporated into modified (C57BL/6 x 129S4/SvJae)F1-derived D34 embryonic stem (ES) cells.
  • mutations:
  • Insertion
  • synonyms:
  • iAMPK<CA>,
  • Col1a1<tm1(tetO-GFP,-PRKAA1*)Rshaw>,
  • Col1a1<tm1(tetO-GFP,-PRKAA1*)Rshaw>,
  • iAMPK<CA>
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0 Mutation Involves

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