| Primary Identifier | MGI:6473223 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cfap276 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology was used to flank exons 3 and 4 with loxP sites and inserted an FRT-flanked neomycin selection cassette downstream of exon 4 but upstream of the second loxP site. Cre-mediated recombination removed exons 3 and 4 and the neo cassette resulting in a frame-shift. Western blotting confirmed absence of protein in central nervous system tissue and sciatic nerve. |
