| Primary Identifier | MGI:6488662 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ankmy2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 36219981 of Chromosome 12 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 36220830. Exon 3 is thus flanked by loxP sites. Subsequent cre-mediated recombination removed the neo cassette and exon 3, creating a reporter knockout allele. Immunoblotting and qRT-PCR confirmed the lack of protein and transcript expression from this allele. |
