| First Author | Nakamura Y | Year | 2016 |
| Journal | J Biol Chem | Volume | 291 |
| Issue | 23 | Pages | 12394-407 |
| PubMed ID | 27044742 | Mgi Jnum | J:235037 |
| Mgi Id | MGI:5792644 | Doi | 10.1074/jbc.M116.724443 |
| Citation | Nakamura Y, et al. (2016) Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged gamma-Aminobutyric Acid Receptor, Type A (GABAA), alpha2 Subunit Knock-in Mouse. J Biol Chem 291(23):12394-407 |
| abstractText | The accumulation of gamma-aminobutyric acid receptors (GABAARs) at the appropriate postsynaptic sites is critical for determining the efficacy of fast inhibitory neurotransmission. Although we know that the majority of synaptic GABAAR subtypes are assembled from alpha1-3, beta, and gamma2 subunits, our understanding of how neurons facilitate their targeting to and stabilization at inhibitory synapses is rudimentary. To address these issues, we have created knock-in mice in which the pH-sensitive green fluorescent protein (GFP) and the Myc epitope were introduced to the extracellular domain of the mature receptor alpha2 subunit (pHalpha2). Using immunoaffinity purification and mass spectroscopy, we identified a stable complex of 174 proteins that were associated with pHalpha2, including other GABAAR subunits, and previously identified receptor-associated proteins such as gephyrin and collybistin. 149 of these proteins were novel GABAAR binding partners and included G-protein-coupled receptors and ion channel subunits, proteins that regulate trafficking and degradation, regulators of protein phosphorylation, GTPases, and a number of proteins that regulate their activity. Notably, members of the postsynaptic density family of proteins that are critical components of excitatory synapses were not associated with GABAARs. Crucially, we demonstrated for a subset of these novel proteins (including cullin1, ephexin, potassium channel tetramerization domain containing protein 12, mitofusin2, metabotropic glutamate receptor 5, p21-activated kinase 7, and Ras-related protein 5A) bind directly to the intracellular domains of GABAARs, validating our proteomic analysis. Thus, our experiments illustrate the complexity of the GABAAR proteome and enhance our understanding of the mechanisms neurons use to construct inhibitory synapses. |
