| First Author | Ratai O | Year | 2019 |
| Journal | Front Mol Neurosci | Volume | 12 |
| Pages | 78 | PubMed ID | 31001084 |
| Mgi Jnum | J:281422 | Mgi Id | MGI:6377977 |
| Doi | 10.3389/fnmol.2019.00078 | Citation | Ratai O, et al. (2019) NCS-1 Deficiency Is Associated With Obesity and Diabetes Type 2 in Mice. Front Mol Neurosci 12:78 |
| abstractText | Neuronal calcium sensor-1 (NCS-1) knockout (KO) in mice (NCS-1(-/-) mice) evokes behavioral phenotypes ranging from learning deficits to avolition and depressive-like behaviors. Here, we showed that with the onset of adulthood NCS-1(-/-) mice gain considerable weight. Adult NCS-1(-/-) mice are obese, especially when fed a high-fat diet (HFD), are hyperglycemic and hyperinsulinemic and thus develop a diabetes type 2 phenotype. In comparison to wild type (WT) NCS-1(-/-) mice display a significant increase in adipose tissue mass. NCS-1(-/-) adipocytes produce insufficient serum concentrations of resistin and adiponectin. In contrast to WT littermates, adipocytes of NCS-1(-/-) mice are incapable of up-regulating insulin receptor (IR) concentration in response to HFD. Thus, HFD-fed NCS-1(-/-) mice exhibit in comparison to WT littermates a significantly reduced IR expression, which may explain the pronounced insulin resistance observed especially with HFD-fed NCS-1(-/-) mice. We observed a direct correlation between NCS-1 and IR concentrations in the adipocyte membrane and that NCS-1 can be co-immunoprecipitated with IR indicating a direct interplay between NCS-1 and IR. We propose that NCS-1 plays an important role in adipocyte function and that NCS-1 deficiency gives rise to obesity and diabetes type 2 in adult mice. Given the association of altered NCS-1 expression with behaviorial abnormalities, NCS-1(-/-) mice may offer an interesting perspective for studying in a mouse model a potential genetic link between some psychiatric disorders and the risk of being obese. |
