| Primary Identifier | MGI:6489595 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Carns1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was introduced into intron 2 and a loxP and FRT site flanked neomycin resistance gene cassette downstream of exon 10. The neo cassette was removed through subsequent flp-mediated recombination; after that, exons 3-10 were deleted through cre-mediated recombination, leaving exons 1, 2 and the duplicated 3' end of exon 10. Northern and Western blot experiments confirmed the absence of mRNA and protein expression from this allele. |
