| First Author | Huszthy PC | Year | 2019 |
| Journal | Proc Natl Acad Sci U S A | Volume | 116 |
| Issue | 51 | Pages | 25850-25859 |
| PubMed ID | 31796587 | Mgi Jnum | J:282821 |
| Mgi Id | MGI:6383247 | Doi | 10.1073/pnas.1902836116 |
| Citation | Huszthy PC, et al. (2019) B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells. Proc Natl Acad Sci U S A 116(51):25850-25859 |
| abstractText | The B cell receptors (BCRs) for antigen express variable (V) regions that are enormously diverse, thus serving as markers on individual B cells. V region-derived idiotypic (Id) peptides can be displayed as pId:MHCII complexes on B cells for recognition by CD4(+) T cells. It is not known if naive B cells spontaneously display pId:MHCII in vivo or if BCR ligation is required for expression, thereby enabling collaboration between Id(+) B cells and Id-specific T cells. Here, using a mouse model, we show that naive B cells do not express readily detectable levels of pId:MHCII. However, BCR ligation by Ag dramatically increases physical display of pId:MHCII, leading to activation of Id-specific CD4(+) T cells, extrafollicular T-B cell collaboration and some germinal center formation, and production of Id(+) IgG. Besides having implications for immune regulation, the results may explain how persistent activation of self-reactive B cells induces the development of autoimmune diseases and B cell lymphomas. |
