| Primary Identifier | MGI:6506427 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells = BL1 (BALB/c). |
| molecularNote | The targeting vector replaced the D-Q52*01-J4 with the B1-8 promoter and the modified V-region of the M315 myeloma protein heavy chain (generated from the MOPC315 cell line) in which the Vlambda2 exon 2 was replaced with a synthetic Vlambda315 exon 2 containing the modification YST to FRN (residues 94-96; TACAGCACC to TTCAGAAAC) and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. |
