| Primary Identifier | MGI:6492300 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Prph2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A G to A point mutation was introduced in exon 2 to create a cysteine to tyrosine substitution at residue 213 (p.C213Y) in the encoded protein. In addition a loxP/FRT flanked neomycin cassette was inserted 882 bp 3' to a second silent mutation (G to A) in exon 2 to introduce a restriction site for genotyping. Flp-mediated recombination removed the Neo cassette. Levels of mutant transcript in homozygotes are similar to wild-type levels, however retinas have only approximately 10% of wild-type levels of protein. |
