| First Author | Zhang Z | Year | 2022 |
| Journal | J Genet Genomics | Volume | 49 |
| Issue | 12 | Pages | 1093-1100 |
| PubMed ID | 36028133 | Mgi Jnum | J:338265 |
| Mgi Id | MGI:7510292 | Doi | 10.1016/j.jgg.2022.08.001 |
| Citation | Zhang Z, et al. (2022) Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations. J Genet Genomics 49(12):1093-1100 |
| abstractText | It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs: gCap (general capillary) and aCap (aerocyte). They are identified by two unique markers, respectively: plasmalemmal vesicle-associated protein (PLVAP) and carbonic anhydrase IV (CAR4). Here, we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER, which genetically target gCap and aCap, respectively. Induced by tamoxifen, the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP(+) gCap and CAR4(+) aCap, respectively, in the lungs. These two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP(+) and CAR4(+) cells in lung homeostasis, injury and repair. |
