| Primary Identifier | MGI:6704920 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Tert |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting vector was generated by inserting a codon-optimized intron-CreERT2-NeoR cassette into the endogenous translational start site of Tert in the BAC (RP24-342O18) via recombineering. The targeting vector was linearized and electroporated into JM8/F6 mouse ES cells. Correctly targeted ES clones were selected by Southern blotting and karyotypes, and then injected into ICR/CD-1 blastocysts to generate the knock-in line. |
