| First Author | Gómez HF | Year | 2021 |
| Journal | BMC Res Notes | Volume | 14 |
| Issue | 1 | Pages | 23 |
| PubMed ID | 33436073 | Mgi Jnum | J:300181 |
| Mgi Id | MGI:6501615 | Doi | 10.1186/s13104-020-05436-0 |
| Citation | Gomez HF, et al. (2021) Morphological study of embryonic Chd8(+/-) mouse brains using light-sheet microscopy. BMC Res Notes 14(1):23 |
| abstractText | OBJECTIVE: Autism spectrum disorder (ASD) encompasses a group of neurodevelopmental conditions that remain poorly understood due to their genetic complexity. CHD8 is a risk allele strongly associated with ASD, and heterozygous Chd8 loss-of-function mice have been reported to exhibit macrocephaly in early postnatal stages. In this work, we sought to identify measurable brain alterations in early embryonic development. RESULTS: We performed light-sheet fluorescence microscopy imaging of N-cadherin stained and optically cleared Chd8(+/-) and wild-type mouse brains at embryonic day 12.5 (E12.5). We report a detailed morphometric characterization of embryonic brain shapes and cortical neuroepithelial apical architecture. While Chd8(+/-) characteristic expansion of the forebrain and midbrain was not observed this early in embryogenesis, a tendency for a decreased lateral ventricular sphericity and an increased intraocular distance in Chd8(+/-) brains was found compared to controls. This study advocates the use of high-resolution microscopy technologies and multi-scale morphometric analyses of target brain regions to explore the etiology and cellular basis of Chd8 haploinsufficiency. |
