| Primary Identifier | MGI:6693435 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Gabra5 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The allele was designed to introduce a glutamic acid-to-cysteine mutation at codon 125 (E152C counting from the start codon; or E125C in the mature peptide sequence) into exon 5 of the gene. The targeting vector also includes a loxP-flanked neomycin selection cassette upstream of exon 5, as well as a C-to-T silent mutation in the exon 5 DNA sequence (to create a HindIII site upstream of the mutation for genotyping). Cre-mediated recombination removed the floxed neo cassette. |
