| Primary Identifier | MGI:6704902 | Allele Type | Endonuclease-mediated |
| Attribute String | Inducible, Recombinase | Gene | Gls2 |
| Strain of Origin | C57BL/6J | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the glutaminase 2 (liver, mitochondrial) locus (Gls2) on chromosome 10. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission. |
