| Primary Identifier | MGI:6711468 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gmppa |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Mutant cell line EPD0621_7_G03 was used to create mutant mice which demonstrated germline transmission (J:305791). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 75414469 of Chromosome 1 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 75415288. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in exon 5 removal and a knockout mouse. Immunoblot analysis confirmed absence of protein. |
