| Primary Identifier | MGI:6718443 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified isoform(s) | Gene | Cryba1 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated a single nucleotide replacement of A to G in the first start codon (ATG to GTG) resulting in a knockdown of the betaA3 isoform. A silent mutation (ACC to ACG) was also introduced to prevent the binding and re-cutting of the sequence by gRNA after homology-directed repair. Western blot analysis confirmed the absence of the betaA3 isoform but also upregulation of the betaA1 isoform level is astrocytes. |
