| Primary Identifier | MGI:6715267 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pah |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated a G to T change (in codon 7; GAG to TAG) to create a stop codon in exon 1 and a premature transcript. Two founders (#5329 and 5349) were generated but line 5349 was selected for analysis. qRT-PCR and Western blot confirmed the absence of transcript and protein in the liver. |
