Primary Identifier | MGI:6726556 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cplx1 |
Transmission | Germline | Strain of Origin | 129P2/Ola |
Is Recombinase | false | Is Wild Type | false |
molecularNote | By homologous recombination in embryonic stem cells (ES), two loxP sites and a neo cassette were introduced flanking the exon 2, one at 212 bp upstream of exon 2 and another at 53 bp downstream of the exon 2 after the Neomycin resistance cassette, which was flanked by two FRT sites. The floxed exon 2 and neo cassette were removed by breeding with a cre-deleter constituting a Cplx1 null allele. |