Primary Identifier | MGI:6720253 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Npvf |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | Using homologous recombination in mouse embryonic stem cells, an internal ribosomal entry sequence (IRES) linked to an improved cre recombinase gene and an FRT-flanked neomycin resistance gene was targeted into the 3' untranslated region immediately downstream of the termination codon within the Npvf gene. FLPe-deleter mice were crossed to remove the FRT-flanked neomycin selection cassette. |