| Primary Identifier | MGI:6766467 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gmeb2 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTGACGAGTCTGAGATGAT and CCAAGAATCCGGTAGAGAAC, which resulted in a 363 bp deletion beginning at Chromosome 2 position 180,902,064 bp and ending after 180,902,426 bp (GRCm39/mm39). This mutation deletes ENSMUSE00001242983 (exon 5) and 259 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 119 and early truncation 13 amino acids later. |
