| First Author | Taniguchi M | Year | 2023 |
| Journal | Mucosal Immunol | Volume | 16 |
| Issue | 5 | Pages | 624-641 |
| PubMed ID | 37385587 | Mgi Jnum | J:341473 |
| Mgi Id | MGI:7511648 | Doi | 10.1016/j.mucimm.2023.06.004 |
| Citation | Taniguchi M, et al. (2023) Sialylation shapes mucus architecture inhibiting bacterial invasion in the colon. Mucosal Immunol |
| abstractText | In the intestine, mucin 2 (Muc2) forms a network structure and prevents bacterial invasion. Glycans are indispensable for Muc2 barrier function. Among various glycosylation patterns of Muc2, sialylation inhibits bacteria-dependent Muc2 degradation. However, the mechanisms by which Muc2 creates the network structure and sialylation prevents mucin degradation remain unknown. Here, by focusing on two glycosyltransferases, St6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6) and beta-1,3-galactosyltransferase 5 (B3galt5), mediating the generation of desialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity. The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation. Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of desialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD. In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded. Thus, sialylation mediates the negative charging of Muc2 and facilitates the formation of the mucin network structure, thereby inhibiting bacterial invasion in the colon to maintain gut homeostasis. |
