| Primary Identifier | MGI:6810174 | Allele Type | Targeted |
| Attribute String | Epitope tag | Gene | Itk |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence for a Gly-Ser-Gly spacer, a OneâSTrEPâtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3â² end of the gene coding sequence. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. |
