| Primary Identifier | MGI:6763061 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase | Gene | Tg(Mchr1-cre/ERT2)3Nbe |
| Strain of Origin | Not Specified | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A cre/ERT2 expression sequence was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting Mchr1-cre/ERT2 BAC transgene was purified for subsequent male pronuclear injection to establish transgenic founder animals. Two lines 2 and 3 were generated with similar expression patterns. . |
