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Allele : Gt(ROSA)26Sor<tm11.1(Cas9)Npa> gene trap ROSA 26, Philippe Soriano; targeted mutation 11.1, Novartis Pharma AG
Primary Identifier  MGI:6724105 Allele Type  Targeted
Attribute String  Endonuclease, Epitope tag Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  C57BL/6J
Is Recombinase  false Is Wild Type  false
molecularNote  By homologous recombination in the C57BL/6J ES cells, a SA-3xFLAG-NLS-SpCas9-NLS-polyA-loxP-HSVtk-Neo-loxP cassette was inserted into the Gt(ROSA)26Sor locus. Targeted ES cells were used for blastocyst injection to obtain chimeric mice. The loxP-HSVtk-Neo-loxP cassette was removed by mating with cre deleter mice.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26 <tm7<1-5C>Npa,
  • Rosa26-SpCas9,
  • Rosa26 <tm7<1-5C>Npa,
  • Rosa26-SpCas9
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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3 Publication categories

Trail: Allele




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