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Allele : Col1a1<tm1(tetO-cas9*)Jnxu> collagen, type I, alpha 1; targeted mutation 1, Jian Xu
Primary Identifier  MGI:6835795 Allele Type  Targeted
Attribute String  Endonuclease, Epitope tag, Inducible Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The plasmid contains a splice acceptor-polyA sequence and the tetracycline responsive element (tetO) upstream of the dCas9-KRAB fusion gene replacing the FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus. The dCas9/KRAB fusion gene sequence (dK) is composed of a human codon-optimized, nuclease-deficient Streptococcus pyogenes Cas9 protein synthetic DNA, an HA tag, and two copies of a nuclear localization sequence (NLS), and the repressive chromatin modifier domain mRNA from the Kruppel associated box (KRAB) domain of the human zinc-finger protein 10 gene (ZNF10)
  • mutations:
  • Insertion
  • synonyms:
  • dCas9-KRAB,
  • dCas9-KRAB
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1 Feature

Trail: Allele

Genome

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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