| First Author | Lee Y | Year | 2015 |
| Journal | Cell Rep | Volume | 13 |
| Issue | 2 | Pages | 223-33 |
| PubMed ID | 26440898 | Mgi Jnum | J:312451 |
| Mgi Id | MGI:6790396 | Doi | 10.1016/j.celrep.2015.09.005 |
| Citation | Lee Y, et al. (2015) p62 Plays a Specific Role in Interferon-gamma-Induced Presentation of a Toxoplasma Vacuolar Antigen. Toxoplasma Vacuolar Antigen 13(3):223-33 |
| abstractText | Also known as Sqstm1, p62 is a selective autophagy adaptor with a ubiquitin-binding domain. However, the role of p62 in the host defense against Toxoplasma gondii infection is unclear. Here, we show that interferon γ (IFN-γ) stimulates ubiquitin and p62 recruitment to T. gondii parasitophorous vacuoles (PVs). Some essential autophagy-related proteins, but not all, are required for this recruitment. Regardless of normal IFN-gamma-induced T. gondii clearance activity and ubiquitination, p62 deficiency in antigen-presenting cells (APCs) and mice diminishes the robust IFN-gamma-primed activation of CD8(+) T cells that recognize the T. gondii-derived antigen secreted into PVs. Because the expression of Atg3 and Irgm1/m3 in APCs is essential for PV disruption, ubiquitin and p62 recruitment, and vacuolar-antigen-specific CD8(+) T cell activation, IFN-gamma-mediated ubiquitination and the subsequent recruitment of p62 to T. gondii are specifically required for the acquired immune response after PV disruption by IFN-gamma-inducible GTPases. |
