| Primary Identifier | MGI:6790470 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Casp8 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Cysteine codon 362 (TGC) was targeted for change to serine (AGC)(p.C362S) with an sgRNA (targeting CACCGTTTCATTCAGGCTTGCCA) and an ssODN template (CACTGGTTCAAAGTGCCCTTCCCTGTCTGGGAAACCCAAGATCTTTTTCATTCAGGCTAGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTTCGAGCAACAGAAC) using CRISPR/Cas9 technology. The mutation renders the encoded peptide enzymatically inactive. |
