| Primary Identifier | MGI:6840220 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout, Reporter | Gene | Cx3cr1 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing is used to target two sites in exon 2. Double stranded oligonucleotide donor DNAs are created to replace the first 130 amino acids of the endogenous Cx3cr1 locus with Dendra2 (a green to red irreversibly photoswitchable monomeric fluorescent protein derived from octocoral Dendronephthya sp.), an SV40 nuclear localization signal, and a polyadenylation sequence. |
