| Primary Identifier | MGI:6765364 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Tlk2 |
| Transmission | Germline | Strain of Origin | C57BL/6 x 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector from IKMC Project 79307 was used to generate this allele. The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 11. The critical exon 11 is thus flanked by loxP sites. Flp-mediated recombination deleted the lacZ and neomycin resistance gene leaving exon 11 floxed. |
