Primary Identifier | MGI:6827287 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Mirc33 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop.The allele has the Mirc33 locus with loxP sites flanking the critical region for its functions, such that cre mediated recombination would produce null/knock out allele. |