| Primary Identifier | MGI:6759474 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, Null/knockout, Recombinase | Gene | Adgra3 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NTac)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting vector contains a human diphtheria toxin receptor gene (HBEGF) fused to an enhanced green fluorescent protein (EGFP), a self-cleaving peptide T2A, an FRT-flanked neomycin cassette and cre/ERT2 inserted immediately after the first methionine codon. The construct places the diphtheria toxin receptor, EGFP, and cre/ERT2 under the control of the Adgra3 promoter, and excises 502 bp encompassing 221 bp of exon 1 and part of the following intron to generate a knockout. Mice treated with diptheria toxin die due to expression of HBEGF in vital organs. EGFP is not detected in living cells by immunofluorescence or FACS. Flp-mediated recombination removed the FRT-flanked neo cassette. |
