| Primary Identifier | MGI:6761276 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Constitutively active | Gene | Tg(Gt(ROSA)26Sor-DAAM1*))#Jwng |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The transgene consists of 206 kb BAC clone RP24-85L15 that contains the mouse Rosa26 genomic region with inserted ÎDAD, which encodes a constitutively active form of human DAAM1 mutant into the intron 2 of Rosa26. A floxed cassette consisted of three tandem polyadenylation signals was placed in front of ÎDAD so that ÎDAD transcription can only be induced only upon Cre-mediated excision of the 3x poly(A) cassette. A CAG promoter was placed upstream of the floxed 3x poly(A) cassette to enhance transcription, and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was added downstream of ÎDAD to increase mRNA stability. |
